hpac pancreatic cancer cell line  (ATCC)

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Micrographs at the phase contrast microscope showing cell morphology of PDAC cells grown in 2D monolayers and 3D spheroids. While HPAC and BxPC-3 show a more epithelial phenotype and form spheroids containing densely packed cells, MIA PaCa-2 cells on 2D monolayers reveal the presence on both epithelial and mesenchymal cells, and their spheroids are less densely packed. Original magnification 10×.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Microscopy

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Immunofluorescence analysis for E-cadherin (green) and actin cytoskeleton (red) in PDAC cells cultured in 2D monolayers and 3D spheroids. The three PDAC cells exhibited an evident cortical actin, but E-cadherin immunoreactivity at cell membrane was detectable only in HPAC and BxPC-3 cells. Original magnification: 40×.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Immunofluorescence, Cell Culture, Membrane

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Representative Western blot showing E-cadherin expression in whole cell lysates of HPAC ( A ) and BxPC-3 cells ( B ). Bar graphs showing E-cadherin protein levels after densitometric analysis of immunoreactive bands in HPAC ( C ) and BxPC-3 cells ( D ). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Western Blot, Expressing

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Representative Western blot showing VNC expression in whole cell lysates of HPAC ( A ), BxPC-3 ( B ) and MIA PaCa-2 cells ( C ). Bar graphs showing E-cadherin protein levels after densitometric scanning of immunoreactive bands in HPAC ( D ), BxPC-3 ( E ) and MIA PaCa-2 cells ( F ). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Western Blot, Expressing

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Bar graphs showing, respectively, Slug and Snail gene expression in HPAC ( A , D ), BxPC-3 ( B , E ) and MIA PaCa-2 cells ( C , F ) assessed by real-time PCR. Data were normalized on GAPDH gene expression and are expressed as mean ± SD for at least three independent experiments. ** p < 0.01.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Immunofluorescence analysis for YAP/TAZ (green) in PDAC cells cultured in 2D monolayers and 3D spheroids. The merged micrographs show that HPAC and BxPC-3 cells grown in 2D monolayers exhibited a more nuclear immunolabeling compared to 3D spheroids (see arrows), having a predominantly YAP/TAZ cytoplasmic localization. Conversely, in MIA PaCa-2 cells YAP/TAZ immunoreactivity was mostly cytoplasmic in both 2D and 3D cell cultures. Original magnification: 40×.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Immunofluorescence, Cell Culture, Immunolabeling

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Representative Western blot showing YAP and p-YAP expression in whole cell lysates of HPAC ( A ), BxPC-3 ( E ) and MIA PaCa-2 cells ( I ). Bar graphs showing YAP, p-YAP and the YAP/p-YAP ratio, respectively, in HPAC ( B – D ), BxPC-3 ( F – H ) and MIA PaCa-2 cells ( J – L ). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Western Blot, Expressing

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Immunofluorescence analysis for p-YAP (green) in PDAC cells cultured in 2D monolayers and 3D spheroids. The micrographs show that in HPAC and BxPC-3 cells p-YAP expression is mostly cytoplasmic in both 2D and 3D cell cultures. By contrast, MIA PaCa-2 cells grown in 2D monolayer revealed a different pattern of expression. Indeed, the more fibroblastoid subpopulation is characterized by a lower p-YAP expression, whereas the more epithelial cells revealed a more intense and cytoplasmic expression, according to the different cell adhesion on the plastic substrate. Original magnification: 40×.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Immunofluorescence, Cell Culture, Expressing

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: Bar graphs showing, respectively, CTGF, CYR61 and LATS1 gene expression in HPAC ( A – C ), BxPC-3 ( D – F ) and MIA PaCa-2 cells ( G – I ) assessed by real-time PCR. Data were normalized on GAPDH gene expression and are expressed as mean ± SD for at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction

Journal: Cells

Article Title: Mechanical Cues, E-Cadherin Expression and Cell “Sociality” Are Crucial Crossroads in Determining Pancreatic Ductal Adenocarcinoma Cells Behavior

doi: 10.3390/cells11081318

Figure Lengend Snippet: ( A ) Representative SDS-zymography showing MMP-2 and MMP-9 activity assayed in serum-free cell supernatants of PDAC cells. Bar graphs showing MMP-2 and MMP-9 activity after densitometric analysis of lytic bands, respectively, in HPAC ( B , E ), BxPC-3 ( C , F ) and MIA PaCa-2 cells ( D , G ). Data are expressed as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The pancreatic cancer cell lines HPAC, BxPC-3 and MIA PaCa-2 from pancreatic ductal adenocarcinoma (PDAC) were obtained from the American Type Culture Collection (American Type Culture Collection, ATCC) (Manassas, VA, USA).

Techniques: Zymography, Activity Assay